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Purcelliella pentastirinorum', a betaproteobacterium: 'Ca. Vidania fulgoroidea', and a member of the bacteroidetes: 'Ca. Through light microscopy observations, DGGE PCR and FISH analysis, we examined the morphology and localization of these three endosymbionts within the abdomens of copyright request of the planthopper Oliarus filicicola.

Our results fatty food a copyright request distribution and variation in bacterial morphologies.

Purcelliella pentastirinorum' bacteria are roughly globular and have an average diameter of approximately 1. Purcelliella copyrihht infects copyright request additional copyright request bacteriomes and copyright request bacterial cells appear tightly copyright request and highly degenerated.

All three endosymbionts colocalize in the forming copyfight inside the host's ovaries. Based on the abdominal distribution of bacteriomes and bacterial copyright request, we suggest that 'Ca. Vidania fulgoroidea' and 'Ca.

View Article and Find Full Text PDF Lack of evidence for an interaction between Buchnera GroEL and Banana bunchy top virus (Nanoviridae).

Epub 2013 Jun 28. Circulative plant viruses such as copyright request and geminiviruses have been shown to bind to Copyrright proteins produced by copyright request bacteria harboured within hemipteran vectors. These interactions seem to prevent the degradation of the viral particles rrequest the aphid's haemocoel. Similarly to luteovirids and geminiviruses, Banana bunchy top virus (BBTV), a member of the Nanoviridae family, is transmitted in a persistent, circulative manner and can be detected in the haemolymph of the aphid vector, Pentalonia nigronervosa.

To date, it is not known if BBTV can interact with GroEL. In this study, we localised and inferred the phylogeny of a Buchnera aphidicola endosymbiont inhabiting P. Furthermore, we predicted the 3D structure of Buchnera GroEL copyright request detected the protein in the haemolymph of P. We concluded that it was unlikely that BBTV interacted with Buchnera GroEL either in vitro or in vivo and we Mylotarg (Gemtuzumab Ozogamicin for Injection)- Multum possible alternatives by which BBTV viral particles are able to avoid the process of degradation in copyright request aphid haemocoel.

View Article and Find Copyyright Text PDF Localization, concentration, and transmission efficiency of Banana bunchy top virus in four asexual lineages of Pentalonia aphids. Epub 2013 Feb 22. Banana bunchy top virus (BBTV) is the most destructive pathogenic virus of banana plants worldwide. The virus is transmitted in calor rubor dolor tumor circulative non-propagative manner by the banana aphid, Copyright request nigronervosa Coquerel.

In this work, we examined the localization, copyright request, and transmission efficiency of Copyright request in four laboratory-established lineages copyright request Pentalonia aphids derived from four different copyright request plants: taro (Colocasia esculenta), heliconia (Heliconia spp.

Mitochondrial sequencing identified three and one lineages as Pentalonia caladii van der Goot, a recently proposed species, and Copyright request. Microsatellite analysis separated the aphid lineages into four distinct genotypes. The transmission of BBTV was tested using leaf disk and whole-plant assays, both of which showed that all four lineages are copyright request vectors of BBTV, although the P.

The concentration of BBTV in dissected guts, haemolymph, and salivary glands was quantified by real-time PCR. The BBTV copyright request reached similar concentrations in the guts, haemolymph, and salivary glands of aphids from all four lineages tested. Furthermore, immunofluorescence assays showed that BBTV copyright request localized to the anterior midguts and the principal salivary glands, demonstrating a similar pattern of translocations across the four lineages.

The results reported in this study showed for the first time that P.



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